Use of the ultracentrifuge vertical rotor in the detection of rubella-specific IgM on a sucrose density gradient.
نویسندگان
چکیده
A comparison was made of the performance of swing-out and vertical ultracentrifuge rotors in the detection of rubella-specific IgM on a sucrose density gradient. Tests were performed on 30 sera, of which 11 were found to contain rubella-specific IgM by both methods. The centrifugation time for the swing-out rotor was 16 hours at 35,000 rpm. This was reduced to 2 hours using the vertical rotor at 50,000 rpm. Routine use of the vertical rotor would allow a reduction in the time taken to perform the test, increase the number of sera tested each time, and reduce wear on the ultracentrifuge.
منابع مشابه
Reorienting gradient centrifugation in the determination of rubella specific IgM.
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عنوان ژورنال:
- Journal of clinical pathology
دوره 32 9 شماره
صفحات -
تاریخ انتشار 1979